Composite

Part:BBa_K1326004:Design

Designed by: Leah Simmons   Group: iGEM14_Macquarie_Australia   (2014-10-08)


Plac + ChlI1 + ChlD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 2666
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 439
    Illegal BglII site found at 1095
    Illegal BglII site found at 1290
    Illegal BglII site found at 3371
    Illegal BamHI site found at 487
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1946
    Illegal NgoMIV site found at 2765
    Illegal NgoMIV site found at 3307
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimisation and attachment of BioBrick prefix and suffix.


Source

Chlamydomonas reinhardtii

References